Journal: PLOS ONE

Article Title: Rapid and sensitive detection of gram-negative bacteria using surface-immobilized polymyxin B

doi: 10.1371/journal.pone.0290579

Figure Lengend Snippet: a) Schematic of the microfluidic chip with two independent channels. Dimensions, injection hole, vent hole, and dotting points where PMB was immobilized are indicated. b) Relationship between the number of cells and FITC intensities analyzed in the microfluidic chip.

Article Snippet: The microfluidic chip was prepared with top and bottom plates, and fabricated by injecting polymethyl methacrylate (PMMA) to a mold [ ] in a local company (NanoEnTek, Seoul, Korea).

Techniques: Injection

Journal: Biosensors & Bioelectronics

Article Title: A rapid quantitative on-site coronavirus disease 19 serological test

doi: 10.1016/j.bios.2021.113406

Figure Lengend Snippet: Schematics on the on-site quantitative point-of-care microfluidic assay for SARS-CoV-2. A ) Schematic of the microfluidic chip and fluorescence reader. The reader has a display and vent controller for flow digitization, as well as optical unit for detecting fluorescent signals. B) Schematic of microfluidic chip fabrication with NI and NI-driven filling mechanism. C) NI-driven flow digitized by vent control. In 1 and 3, the vent was closed to stop the flow for reaction. In 2 and 4, the vent was open to allow the sample flow to the reaction zone (2) and fully removed for washing (4). D) Process chart for flow digitization, consisting of a camera, controller, and micromotor. E) Schematic of the microfluidic chip to detect anti-Cov19 IgM or anti-Cov19 IgG. The chip consisted of five parts, i) sample inlet (yellow box), ii) conjugated zone (green box) with dots of anti-IgM or anti-IgG conjugated fluoresce beads (FB), iii) test zone (red box) with dots of SARs-CoV2 antigen-immobilized IgG/IgM, iv) control zone (blue box) with dots immobilized anti-FBs as a control, and v) vents. . (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The NI-driven flow stopped at a desired position and duration by controlled vent opening/closing, providing sufficient reaction time only with the fixed sample volume and significantly enhancing the limit of detection (LOD) ( ; ) , Top and bottom substrates of the microfluidic chip were fabricated by injection moulding of Polymethyl methacrylate (PMMA) (Incyto, Korea) and bonded by acetone injection using an in-house plate press machine with a pressure of 0.5 MPa (Absology, Korea).

Techniques: Fluorescence, Control

Journal: Biosensors & Bioelectronics

Article Title: A rapid quantitative on-site coronavirus disease 19 serological test

doi: 10.1016/j.bios.2021.113406

Figure Lengend Snippet: Performance of the developed microfluidic assay A) Cut-off index(COI) reproducibility of the assay on concentration. B) COI of IgM/IgG for negative control using sera collected prior to the COVID-19 pandemic. C) IgG (S/COI) versus IgM (S/COI) plot showing the sensitivity (91.67%) and specificity (100%) of the developed microfluidic assay. D) Specificity versus sensitivity plot of other US Food and Drug Administration serological tests (LFA, CLIA, ELISA, Microsphere immunoassay). Additional details are provided in .

Article Snippet: The NI-driven flow stopped at a desired position and duration by controlled vent opening/closing, providing sufficient reaction time only with the fixed sample volume and significantly enhancing the limit of detection (LOD) ( ; ) , Top and bottom substrates of the microfluidic chip were fabricated by injection moulding of Polymethyl methacrylate (PMMA) (Incyto, Korea) and bonded by acetone injection using an in-house plate press machine with a pressure of 0.5 MPa (Absology, Korea).

Techniques: Concentration Assay, Negative Control, Enzyme-linked Immunosorbent Assay

Journal: Biosensors & Bioelectronics

Article Title: A rapid quantitative on-site coronavirus disease 19 serological test

doi: 10.1016/j.bios.2021.113406

Figure Lengend Snippet: Schematics on the on-site quantitative point-of-care microfluidic assay for SARS-CoV-2. A ) Schematic of the microfluidic chip and fluorescence reader. The reader has a display and vent controller for flow digitization, as well as optical unit for detecting fluorescent signals. B) Schematic of microfluidic chip fabrication with NI and NI-driven filling mechanism. C) NI-driven flow digitized by vent control. In 1 and 3, the vent was closed to stop the flow for reaction. In 2 and 4, the vent was open to allow the sample flow to the reaction zone (2) and fully removed for washing (4). D) Process chart for flow digitization, consisting of a camera, controller, and micromotor. E) Schematic of the microfluidic chip to detect anti-Cov19 IgM or anti-Cov19 IgG. The chip consisted of five parts, i) sample inlet (yellow box), ii) conjugated zone (green box) with dots of anti-IgM or anti-IgG conjugated fluoresce beads (FB), iii) test zone (red box) with dots of SARs-CoV2 antigen-immobilized IgG/IgM, iv) control zone (blue box) with dots immobilized anti-FBs as a control, and v) vents. . (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The NI-driven flow stopped at a desired position and duration by controlled vent opening/closing, providing sufficient reaction time only with the fixed sample volume and significantly enhancing the limit of detection (LOD) ( ; ) , Top and bottom substrates of the microfluidic chip were fabricated by injection moulding of Polymethyl methacrylate (PMMA) (Incyto, Korea) and bonded by acetone injection using an in-house plate press machine with a pressure of 0.5 MPa (Absology, Korea).

Techniques: Fluorescence, Control

Journal: Biosensors & Bioelectronics

Article Title: A rapid quantitative on-site coronavirus disease 19 serological test

doi: 10.1016/j.bios.2021.113406

Figure Lengend Snippet: Performance of the developed microfluidic assay A) Cut-off index(COI) reproducibility of the assay on concentration. B) COI of IgM/IgG for negative control using sera collected prior to the COVID-19 pandemic. C) IgG (S/COI) versus IgM (S/COI) plot showing the sensitivity (91.67%) and specificity (100%) of the developed microfluidic assay. D) Specificity versus sensitivity plot of other US Food and Drug Administration serological tests (LFA, CLIA, ELISA, Microsphere immunoassay). Additional details are provided in .

Article Snippet: The NI-driven flow stopped at a desired position and duration by controlled vent opening/closing, providing sufficient reaction time only with the fixed sample volume and significantly enhancing the limit of detection (LOD) ( ; ) , Top and bottom substrates of the microfluidic chip were fabricated by injection moulding of Polymethyl methacrylate (PMMA) (Incyto, Korea) and bonded by acetone injection using an in-house plate press machine with a pressure of 0.5 MPa (Absology, Korea).

Techniques: Concentration Assay, Negative Control, Enzyme-linked Immunosorbent Assay

Journal: Biosensors

Article Title: Integrated Microfluidic Devices Fabricated in Poly (Methyl Methacrylate) (PMMA) for On-site Therapeutic Drug Monitoring of Aminoglycosides in Whole Blood

doi: 10.3390/bios9010019

Figure Lengend Snippet: ( a ) Photograph image of the double-V hybrid PMMA/adhesive tape device filled with green food dye. Scale bar = 10 mm. ( b ) Zoomed in microscope image of the offset double-V channel filled with green food dye (white box in panel a). Scale Bar = 100 µm. ( c ) Schematic of the microfluidic device (dimension not to scale) indicating voltages and terminating current used for generation of the extraction nanojunction and ( d ) the desalting nanojunction. ( e ) Injection voltages (60 s) to extract, concentrate, and desalt injected plug (blue) of small molecules (ROX and fluorescein). ( f ) Separation voltages (180 s) for electrophoretic separation. Buffer (B), Buffer Waste (BW), Sample (S), and Sample Waste (SW).

Article Snippet: All microfluidic devices were created on a 75 mm × 50 mm × 1.5 mm piece of PMMA (RS Components, Melbourne, Australia) using the following procedures, with a schematic shown in .

Techniques: Adhesive, Microscopy, Extraction, Injection